An Assessment of Recent Advances in the Study of the Chemistry and Biochemistry of Dental Plaque Fluid

This paper discusses key points made during the symposium in the light of work carried out in other laboratories. It is emphasized that the unique importance of plaque fluid is that the net result of chemical changes induced by microbial activ-ity is reflected in this medium, which is in intimate contact with the enamel surface, and that this medium is accessible to chemical and biochemical analyses. However, in order to as-sess the cariogenic potential of plaque, we must consider the properties of both whole plaque and plaque fluid together. Although it is apparent that results of plaque fluid composition are sensitive to both isolation and the storage procedures uti-lized, plaque fluid appears to be a distinct entity within the oral cavity. Technical advances have been made which allow for the determination of the activity of selected ions (hydrogen, calcium, phosphate, potassium, fluoride) in plaque fluid ob-tained from a single site within the mouth. It appears, however, that such data alone may be insufficient to define the cariogenic potential of plaque appropriately. Evidence is presented from which it can be concluded that, with use of pooled samples of plaque obtained from individuals with clear differences in car-ies experience, results on plaque and plaque fluid composition can be obtained which are consistent with noted differences in caries susceptibility. The importance of base production is also discussed, and it is noted that few studies have been carried out to elucidate the role of proteins found in plaque fluid. In conclusion, recent advances in the study of plaque fluid have provided new insights into the mechanism of caries formation which are also germane to the formation of dental calculi