mmunoturbidimetric Assay for a1-Acid Glycoprotein in Serum with a entrifugal Analyzer

his is a simple immunoturbidimetnc method for the rapid sessment of a1-acid glycoprotein in serum with the “Flexi-sm ” microcentrifugal analyzer. Results obtained for a pedi-nc population (n = 95) agree with those by radial immuno-ffusion (x) and yield the regression equation: y = 1.4x + 017 (r = 0.98). Results are printed automatically after a ocedure that takes only about 15 mm. Within-run precision V) ranged from 2.2 to 2.5 % for concentrations of 0.35 to 4 g/L. Day-to-day CV was 4.25 % for an average concentra-n of 0.9 gIL. ddltlonal Keyphrases: orosomucoid- inflammation acute-ase proteins a1-Acid glycoprotein (ai-AG, orosomucoid) is widely de-ermuned in clinical laboratories in conjunction with C-eactive protein to monitor inflammation (1-3). To this end, rapid nephelometric or turbidimetric methods tre needed. We describe here an immunoturbidimetric nethod used with a centrifugal analyzer, in which results ire printed directly within 15 miii. Aaterlals and Methods Reagents. Goat antiserum to human a1-AG (nephelomet