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oocyte FEBS Letters 581 (2007) 663–666Abbreviations: Fructose-2,6-bisP, fructose 2,6-bisphosphatestem from limitations of the methods employed and also from differences in the experimental conditions used (for a review see [6]). However, the influence of the nutritional state as a ma-jor factor involved in the indirect route contribution has been recognized in several studies. As Newgard and coworkers al-ready noted [2], the administration of a large glucose bolus to rats resulting in a portal glucose concentration of 15 mM causes preferential glycogen synthesis through the direct path-way. Also, Landau and Warren [7] reported that the contribu-sults obtained show that the contribution of one pathway or the other to glycogen formation depends indeed on the amount of glucose microinjected into the oocytes. The indirect path-way is the only route operating at low glucose concentrations, while the direct route becomes predominant when intracellular glucose rises above 1 mM. 2. Materials and method