THE MAL YS IS OF NITRO POLYCYCLIC AROMATIC HYDROCARBONS IN DIESEL EXHAUST MD URBM AIRBORNE PARTICULATE SAMPLES By

Analytical methodology has been developed for the determination of nitro polycyclic aromatic hydrocarbons (nitro-PAHs), polycyclic aromatic hydrocarbons (PAHs) and other PAH derivatives in diesel exhaust and urban airborne particulate samples. Samples were.Soxhlet extracted wi th Illethylene " chloride for twenty-four hours and the ' organic" extract filtered to remove particulate matter. This extract was taken through a three stage clean-up procedure. Very'polar materials were initially removed using a silica Sep-Pak. The aromatic compounds were than separated from the aliphatic substances by Sephadex LH-20 chroma-tography. Normal phase ' semi-preparative high performance liquid chromatography was used.for the final fractionation of the aromatic compounds. Three fractions, containing PAHs (Fraction A). ni~ro-PAHs..(Fraction B) and dinitro-PAHs (Fraction t), were collected for further P'.a[lalysis. Two diesel exhaust (Oldsmobile and Peugeot) and four air-borne particulate (Toronto and Hamilton ~ Ontario) samples were taken through thi s procedure