a review and references

For laboratories that are already an-alyzing for theophylline with this pro-cedure, these are the modifications used for the acetaminophen determination. The internal standard is-hydroxy-ethyltheophylline (400 mg/liter in ace-tonitrile). The solvent flow rate is 3.0 ml/min, and the detector sensitivity is set at 0.2 absorbance unit full-scale. The retention times are 3.0 mm for acet-aminophen and 5.3 mm for the internal standard. Serum standards supple-mented with acetaminophen gave a linear and accurate response with con-centrations from 25 to 400 mg/liter. This rapid procedure can be performed with as little as 25 l of sample, which makes it particularly useful in suspected acet-aminophen overdose cases. We have not encountered any interferences from commonly abused drugs, drugs found in acetaminophen preparations, or vita-mins