Specific Subtype of Human Papillomavirus, and Progression of LSIL/CIN1 Lesions First Comparative Study

Objectives: To study and compare the effectiveness of p16INK4a staining and specific human papillomavirus (HPV) subtypes as a prognostic marker in cervical intraepithelial neoplasia grade 1 (CIN1; low-grade squamous intraepithelial lesions). Methods: Sixty-four cervical samples diagnosed as CIN1 and stained with p16INK4a, with HPV status assessed by polymerase chain reaction–direct sequencing. Results: Of the 34 p16INK4a-negative biopsy specimens, 26 regressed, seven persisted, and one progressed. Of the 20 p16INK4a diffusely positive biopsy specimens, seven regressed, eight persisted, and five progressed. Ten biopsy specimens stained positive only in the lower one-third of the sample, of which seven regressed and three persisted. p16INK4a diffusel