POSTER PRESENTATION Open Access Model of dynamics of intracellular chloride based on

Fluorescent indicators are relatively non-invasive probe that allow the measure of ion concentrations in brain slices and neuron cultures using microscopy imaging. Most fluorescent ion indicators bind selectively with a certain ion, causing a decrease in fluorescence in a pro-cess known as quenching. Under steady state conditions, a fluorescence measurement, made at a specific point and time, is directly related to the local ion concentra-tion at the same point and time, typically via the Stern-Volmer relationship. However, this is usually no longer true under the dynamic conditions inside a cell when transmembrane currents are active. While calcium ions (Ca+) dynamics have been well analyzed theoretically [1], chloride ions (Cl-) indicators have been experimen-tally shown to exhibit changes in the timescale of min