Phenotype changes in tumor vessels associated with the progression of hepatocellular carcinoma

Human hepatocellular carcinoma (HCQ is characterized by hypervascularity and tumor stain-ing in angiograms, and tumor angiogenesis is considered indispensable for tumor growth. HCC is also characterized by an obvious multistage process of tumor progression. To find out in which stage of human hepatocarcinogenesis angiogenesis occurs, we have carried out a patho-logical study, of the phenotypic changes in tumor vessels taken from surgically resected liver tumors showing each step of the progression. Eleven early advanced HCCs (advanced HCC com-ponent in early HCC nodule, eAd HCQ, seven early HCCs (eHCQ and six adenomatous hyper-plasias (AH), the non-tumorous liver surrounding each and five normal livers were studied by lectin histochemistry and immunohistochemistry. The sinusoidal endothelial cells from the non-tumorous liver were shown to be negative for UEA-I (Ulex europaeus I), but the endothelial cells from the sinusoidal tumor vessels in advanced HCC components were strongly positive. In AH and eHCC, half the tumors were negative and the other half focally positive. In the early HCC lesion of eAdHCC, the rate of positivity for UEA-I was a little higher than in AH and eHCC but lower than in advanced HCC lesions in eAdHCC. Immunohistochemically, laminin was not detected in the sinusoids in the non-tumorous liver or in the sinusoidal tumor vessels in AH and eHCC. In eAdHCC, however, two early HCC lesions and four advanced HCC lesions were positive. An immunohistochemical examination for muscle actin revealed an increase in arteri-al tumor vessels in six advanced HCC lesions and one early HCC lesion of eAdHCC The results indicate the emergence of UEA-l-positive sinusoidal tumor vessels to be most pronounced dur-ing the progression from eHCC to advanced HCC, and laminin-positive sinusoidal tumor ves-sels or actin-positive arterial tumor vessels to emerge mainly in advanced HCC