Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)

Abstract: This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results:- The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions.- The percentage of germination was 75%.-Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.)