In the past decades, RNA molecules have emerged as important players in numerous cellular pro-cesses. To understand these processes at the mo-lecular and atomic level, large amounts of homogeneous RNA are required for structural, biochemical and pharmacological investigations. Such RNAs are generally obtained from laborious and costly in vitro transcriptions or chemical syn-thesis. In 2007, a recombinant RNA technology has been described for the constitutive production of large amounts of recombinant RNA in Escherichia coli using a tRNA-scaffold approach. We demon-strate a general applicable extension to the described approach by introducing the following im-provements: (i) enhanced transcription of large recombinant RNAs by T7 RNA polymera...
T7 RNA polymerase, DNA dependent RNA polymerase commonly employed in virus molecular research, in vi...
High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: ...
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology ...
In the past decades, RNA molecules have emerged as important players in numerous cellular pro-cesses...
In the past decades, RNA molecules have emerged as important players in numerous cellular pro-cesses...
In the past decades, RNA molecules have emerged as important players in numerous cellular processes....
Background: Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host...
Preparative synthesis of RNA is a challenging task that is usually accomplished using either chemica...
Research on RNA function and therapeutic potential is dominated by the use of chemoengineered RNA mi...
The discovery of functional small noncoding RNAs (ncRNAs), such as microRNAs and small interfering R...
In recent years, it has become clear that RNA molecules are involved in almost all vital cellular pr...
This invention relates to the production of RNA by co-expressing a tRNA ligase and a chimeric RNA mo...
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegan...
International audienceRNA aptamers have several advantages over DNA aptamers due to their propensity...
Reconstitution of protein complexes has been a valuable tool to test molecular functions and to inte...
T7 RNA polymerase, DNA dependent RNA polymerase commonly employed in virus molecular research, in vi...
High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: ...
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology ...
In the past decades, RNA molecules have emerged as important players in numerous cellular pro-cesses...
In the past decades, RNA molecules have emerged as important players in numerous cellular pro-cesses...
In the past decades, RNA molecules have emerged as important players in numerous cellular processes....
Background: Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host...
Preparative synthesis of RNA is a challenging task that is usually accomplished using either chemica...
Research on RNA function and therapeutic potential is dominated by the use of chemoengineered RNA mi...
The discovery of functional small noncoding RNAs (ncRNAs), such as microRNAs and small interfering R...
In recent years, it has become clear that RNA molecules are involved in almost all vital cellular pr...
This invention relates to the production of RNA by co-expressing a tRNA ligase and a chimeric RNA mo...
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegan...
International audienceRNA aptamers have several advantages over DNA aptamers due to their propensity...
Reconstitution of protein complexes has been a valuable tool to test molecular functions and to inte...
T7 RNA polymerase, DNA dependent RNA polymerase commonly employed in virus molecular research, in vi...
High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: ...
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology ...