HEMATOPOIESIS The zebrafish klf gene family

The Krüppel-like factor (KLF) family of genes encodes transcriptional regulatory proteins that play roles in differentiation of a diverse set of cells in mammals. For instance, the founding member KLF1 (also known as EKLF) is required for normal globin production in mammals. Five new KLF genes have been isolated from the zebrafish, Danio rerio, and the structure of their products, their genetic map posi-tions, and their expression during devel-opment of the zebrafish have been charac-terized. Three genes closely related to mammalian KLF2 and KLF4 were found, as was an ortholog of mammalian KLF12. A fifth gene, apparently missing from the genome of mammals and closely related to KLF1 and KLF2, was also identified. Analysis demonstrated the existence of novel conserved domains in the N-termini of these proteins. Developmental expres-sion patterns suggest potential roles for these zebrafish genes in diverse pro-cesses, including hematopoiesis, blood vessel function, and fin and epidermal development. The studies imply a high degree of functional conservation of the zebrafish genes with their mammalian homologs. These findings further the un-derstanding of the KLF genes in verte-brate development and indicate an an-cient role in hematopoiesis for the Krüppel-like factor gene family. (Blood