Genome Sequence of the Polychlorinated-Biphenyl Degrader

Pseudomonas pseudoalcaligenes KF707 is a soil polychlorinated biphenyl (PCB) degrader, able to grow both planktonically and as a biofilm in the presence of various toxic metals andmetalloids. Here we report the genome sequence (5,957,359 bp) of P. pseudoalcaligenesKF707, which provides insights into metabolic degradation pathways, flagellar motility, and chemotaxis. Pseudomonas pseudoalcaligenes KF707 is a robust soil polychlo-rinated biphenyl (PCB) degrader (5) that is able to grow in the presence of various toxic metals andmetalloids (6, 7) as a biofilm, and it has been found to be chemotactically attracted to biphenyl and PCBs (7). Here we present the draft genome sequence of P. pseudoalcali-genes KF707, providing information on the genetic bases for the peculiar physiological aspects and environmental behaviors of this strain, such as chemotaxis, biofilm formation, and metabolic degradation properties. Sequencing technologies used included the 454 Genome Se-quencer FLX system (Roche) and HiSeq2000 (Illumina) (2). Th