High multiplicity infection by HIV-1 in men who have sex with men. PLoS Pathog

Hui Li
Katharine J. Bar
Shuyi Wang
Julie M. Decker
Yalu Chen
Chuanxi Sun
Jesus F. Salazar
Maria G. Salazar
Gerald H. Learn
Charity J. Morgan
Joseph E. Schumacher
Elena E. Giorgi
Tanmoy Bhattacharya
Bette T. Korber
Alan S. Perelson
Joseph J
Myron S. Cohen
Charles B. Hicks
Barton F. Haynes
Martin Markowitz
On F. Keele
Beatrice H. Hahn
George M. Shaw

Publication date

January 2010

Abstract

Elucidating virus-host interactions responsible for HIV-1 transmission is important for advancing HIV-1 prevention strategies. To this end, single genome amplification (SGA) and sequencing of HIV-1 within the context of a model of random virus evolution has made possible for the first time an unambiguous identification of transmitted/founder viruses and a precise estimation of their numbers. Here, we applied this approach to HIV-1 env analyses in a cohort of acutely infected men who have sex with men (MSM) and found that a high proportion (10 of 28; 36%) had been productively infected by more tha