Partial purification, immobilization and preliminary biochemical characterization of lipases from

Copyright © 2013 Ana Lúcia Ferrarezi et al. This is an open access article distributed under the Creative Commons Attribution Li-cense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Lipases have important applications in bio-technological processes, motivating us to pro-duce, purify, immobilize and perform a bio-chemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purifi-cation by gel filtration chromatography in Sephacryl S-100 allowed obtaining a yield of about 85 % and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in it