Title: Breeding for Bruchid Resistance in Common Bean (Phaseolus vulgaris L.): Interspecific Introgression of Lectin-like Seed Proteins from Tepary Bean (P. acutifolius A. Gray), Genetic Control and Bruchid Resistance Characterization.

Interspecific hybridization was initiated between wild P. acutifolius accession G40199 and P. vulgaris to introgress resistance to Acanthoscelides obtectus (bean seed weevil). F1 interspecific hybrids were recovered by embryo rescue and maintained until flowering. Mostly sterile hybrids were backcrossed twice to common bean cultivar ICA Pijao to recover sufficient fertility for the lines to reproduce without assistance. The inheritance of a 33 kDa seed storage protein from accession G40199 was studied in an F2 intraspecific population from a cross of G40199 and a cultivated Brown Tepary accession. G40199 possessed the protein but Brown Tepary did not. The protein was inherited as a single dominant gene in a F2 population of 116 individuals. Following these observations, interspecific hybrids were progeny tested for the introgression of a 33 kDa protein from accession G40199. Backcross interspecific introgression lines were used as bridge parents to transfer the 33 kDa protein into the large red seeded cultivar Rojo adapted to Tanzania, and into a phaseolin null backcross breeding line of Rojo with the objective of introgressing resistance to A. obtectus into a Sub-Saharan Africa variety. Genomic, proteomic an