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circulating in other parts of the world, I present the results obtainedCirculating in São Paulo, Brazil in a previous study conducted in Italy during my stay at the Institute of Oncology, University of Padova, where we analyzed 7 tissue samples obtained from AIDS-related KS patients from São Paulo. To the Editor—It was with interest that I read the paper by Di We detected HHV-8 sequences in all cases, using an outer set of Alberti et al. [1], in which they reported the presence of human primers before using primer-pairs specific for the KS330233 se-herpesvirus 8 (HHV-8) variants in the oral tissues of human immu- quence [7]. Direct sequencing of the amplified products was done, nodeficiency virus (HIV)–infected patients from London. and the results were compared with the sequence described by Using single-strand conformational polymorphism and sequence Chang et al. [2]. A substitution in nucleotide 1033 and, conse-analyses, Di Alberti et al. observed different patterns of DNA gel quently, a change in amino acid 134 of the minor capsid protein migration related to nucleotide substitutions in sequences charac- of KS-associated herpesvirus were detected in all isolates. Another terizing HHV-8 variants. They analyzed sequences corresponding change in nucleotide 1132 that led to an amino acid substitution to 210-bp DNA amplified from open-reading frame (ORF) 26 of at position 167 was also identified in 4 cases. Other base substitu-the HHV-8 genome from 10 oral tissue samples from HIV-infected tions that were silent in amino acid changes were also observed patients, from 5 oral Kaposi’s sarcoma (KS) samples from AIDS among Brazilian HHV-8 isolates and were located at nucleotide