A New Model for Inducing Transient Cerebral Ischemia and Subsequent Reperfusion in Rabbits Without Craniectomy

An artificial ball removable by an attached fiber was injected into the middle cerebral artery (MCA) of 16 rabbits, allowing the study of transient (5, 10,15, or 30 minutes) cerebral ischemia without craniectomy. Measurements of available oxygen (ao2) in the ischemic core (the ventral part of the temporal area) followed by histologic examination verified the embolization. Electroencephalograph-ic power spectra and steady (direct current) potentials were recorded bilaterally on the convexity remote from the actual lesion but still supplied by the MCA. Local cerebral blood flow and ao2 in the border zone between the anterior cerebral artery and the occluded MCA were measured. Embolization caused typical ischemic changes ipsilaterally and alterations characteristic of dias-chisis contralaterally. Extreme border zone hyperemia developed without significant ao2 changes in the same region. Restoration of circulation via the circle of Willis induced gradual normalization. Our model for controlled embolization and recirculation proved suitable for detailed studies of the complex changes in brain function caused by transient ischemia. (Stroke 1988;19:1262-1266) Despite great efforts to clarify the exact patho-mechanism of cerebral ischemia, all patho-physiologic mechanisms involved are not yet known.'-3 For obvious reasons, it would be very important to establish the duration and extent o