Біологічні науки Scientific Journal «ScienceRise » №2/1(7)2015

головного забруднювача повітря оксиду азоту, одержані результати мають значення при проведенні екологічного моніторингу довкілля Ключові слова: оксид азоту, ко-мутаген, аскорбінова кислота, лімфоцити крові, аберації хромосом, проліферативний потенціал Aim. Identify the features of formation of chromosomal instability in lymphocytes of nominally healthy individuals under conditions of combined action of nitrogen oxides (NO) and ascorbic acid (AA), depending on their concentration (study in vitro). Material and methods. Culture of human peripheral blood lymphocytes (PBL) with metaphase analysis of chromosome aberrations. Nitrosated glutathione as the main transport form of NO injected into PBL culture in the concentration range 0,25–0,5–1,0 mmol/ml of blood. As co-mutagen used AA in the concentration range 20,0–40,0–80,0 mg/ml of blood, corresponding therapeutic drug concentrations, as well as 2 and 4 times exceeding its value. Results. Discovered co-mutagenic effect of AA manifested in increasing the overall frequency of chromosomal aberrations in 1.7 and 1.4 times respectively the concentration of NO. The spectrum induced damage presents a whole by chromatid type aberrations that occur mainly by single-strand DNA breaks and kept stable in a numbe