호르 몬 불응 성 전립선 암 환자에 서 Epidermal Growth Factor Receptor 와 HER-2 유전자 의 역할 Role of Epidermal Growth Factor Receptor and the HER-2 Gene in Hormone Refractory Prostate Cancer

Purpose: Amplification and mutation of the epidermal growth factor re-ceptor (EGFR) and HER-2 genes were analyzed in the tissues of hormone refractory prostate cancer (HRPC) patients. Materials and Methods: Gene amplifications of the EGFR and HER-2 gene were analyzed by fluorescence in situ hybridization (FISH) with direct sequencing. Studies were performed on 10 patients; tissues were sampled at the time of initial diagnosis and after the conversion to HRPC (a total of 20 tissue samples). Direct sequencing was performed on exons 18-24 of the EGFR gene and exons 19 and 20 of the HER-2 gene. The ampli-fications and mutations were compared with the clinicopathologic features. Results: Gene amplification of the EGFR gene was observed in 6 (30%) out of 20 samples. A total of six EGFR mutations in exons 18 and 19 were detected in three pairs of tissues (three patients). One patient with a hormone refractory status had a novel deletion mutation in EGFR exon 19. EGFR mutations were associated with the acinar type of prostat