Occurrence of protozoans & their limnological relationships in some ponds of Mathbaria,

Sample collection and identification of protozoans Water samples were collected fortnightly between October 2004 to May 2005 from four water bodies of Mathbaria of Pirojpur district in Bangladesh. For zooplankton sample collection, 100 liters of water were filtered successively through 20 µm mesh nylon nets (Millipore corp., Bedford, MA), and 50 ml of the concentrates were collected initially as a crude measure of zooplankton. From 50 ml concentrates, 10 ml was used for analysis and the samples were immediately preserved in 5% buffered formaldehyde solution. The bottle containing the zooplankton was gently swiveled (clock wise) for equal suspension of zooplankton in the sampled water. By using a clean large mouth 10 ml pipette, 1µl of suspended material was placed in counting cell slot. The counting cell was then covered with a glass slide and examined under a compound binocular microscope at a magnificent of 40 X to 100 X. The Protozoan samples were observed under a compound microscope (Axioskop 40, Japan). For protozoans analysis, heliber bacteria single round cell (Cat no. Z 30000 LOT 11756) was used. The specimens were identified up to genera level. Quantitative analysis was followed by the total count method of Welch (1948). Identification wa