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Click here to view linked References 2 Abstract 26 Insulin-like growth factor-1 and insulin-like growth factor-1 receptor (IGF-1 and 27 IGF-1R) play main roles in vertebrate growth and development. In fish, beside 28 contributing to somatic growth, both molecules exhibit pleiotropic functions. We 29 isolated complete cDNAs sequences encoding for both IGF-1 and IGF-1R in the 30 Chilean flounder by using RT-PCR and rapid amplification of cDNAs ends (RACE) 31 techniques. In addition, we analyzed gene expression in pre-metamorphic larvae and 32 different organs of adult fish through whole mount in situ hybridization and RT-PCR, 33 respectively. The IGF-1 cDNA sequence displays an open reading frame of 558 34 nucleotides, encoding a 185 amino acid preproIGF-1. Moreover, IGF-1R contains an 35 open reading frame spanning 4,239 nucleotides, rendering a 702 amino acid subunit 36 alpha and a 676 amino acid subunit beta. The deduced mature IGF-1 and IGF-1R 37 exhibited high sequence identities with their corresponding orthologs in fishes, specially 3