Species: Mouse

Method of mapping: Zfp15 was localized by haplotype analysis of 94 progeny from an interspecific backcross, (C57BL/6J x M. spre-tus)F 1 x C57BL/6J [1]. Molecular reagents used for mapping: The Zfp15 probe was ob-tained by polymerase chain reaction amplification of a 552-bp fragment from mouse pituitary cDNA with primers designed on the basis of the rat Zfpl5 cDNA sequence [2]. The primers amplify 465 bp of coding sequence, outside of the conserved zinc finger regions, corresponding to the last 154 amino acids, and 87 bp of the 3 ' untranslated region. The RT-PCR product was cloned into the pGEM4Z vector and sequenced toconfirm its correspondence with the rat Zfpl5 cDNA. Allele detection: A BglI polymorphism was detected in mouse genomic DNA with the Zfp15 probe, resulting in an M. spretus-specific restriction fragment of 15 kb and a common restriction fragment of 24 kb. The mapping was confirmed with a BamHI polymorphism, resulting in two M. spretus-specific restriction fragments of 8.9 and 6.6 kb and two common fragments of 18 and 5.5 kb. Correspondence to. " S.A. Campe