Title: Mechanisms of Action by Some Inhibitors of Aflatoxin B1 Carcinoqenesis in Rainbow Trout.

Indole-3-carbinol (I3C) and butylated hydroxyanisole (BHA), anti-carcinogens present in the human diet, were tested for their in vivo and in vitro effect on aflatoxin B,. (AFB1) metabolism, and DMA adduct formation in the rainbow trout. Dietary BHA at either 0.3 or 0.03? had no effect on the hepatic tumor incidence of trout exposed to a 0.5 ppm AFB1 solution as embryos, or when fed prior to and during dietary exposure to 10 ppb AFB... Previous studies have shown 0.1? I3C to inhibit AFB..-induced hepatomas in trout. When fed at 0.2?, I3C produced a 70? reduction in average in vivo hepatic DMA binding of injected AFB,. over a 21 day period compared to controls. A similar study with 0.3? BHA had no effect on AFB..-DNA binding over a 7 day period. One hr incubations of AFB.. with freshly isolated hepatocytes from either BHA-, I3C- or control-fed trout showed no differences in AFB.. metabolism or DNA binding between BHA hepatocytes and controls. However, I3C hepatocytes had 20? less DNA binding with a 2-fol