PyETV: a PyMOL evolutionary trace viewer to analyze functional site predictions in protein complexes

Summary: PyETV is a PyMOL plugin for viewing, analyzing and manipulating predictions of evolutionarily important residues and sites in protein structures and their complexes. It seamlessly cap-tures the output of the Evolutionary Trace (ET) server, namely ranked importance of residues, for multiple chains of a complex. It then yields a high resolution graphical interface showing their distri-bution and clustering throughout a quaternary structure, including at interfaces. Together with other tools in the popular PyMOL viewer, PyETV thus provides a novel tool to integrate evolutionary forces into the design of experiments targeting the most functionally rele-vant sites of a protein. Availability: The PyETV module is written in Python. Installation instructions and video demonstrations may be found at the URL