Distribution of two urinary ribonuclease-like enzymes in human organs and body fluids. J Biochem 99: 17–25. PubMed

1. In order to determine the distribution of two human urinary RNase (RNase Us and RNase Ut,)-like enzymes in human tissues and body fluids, enzyme immuno-assay systems were established using rabbit anti-RNase sera. The sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previ-ously reported. 2. In the enzyme immunoassay, the cross reactivities of anti-RNase UL serum towards RNase Us, bovine kidney RNase Ks, bovine RNase A, and bovine seminal RNase Vg were less than 1 %. The cross reactivity of anti-RNase Us-serum to-wards RNase UL was less than 0.5 % and cross reactivities were minimal for RNase A, RNase K,, and RNase V8. 3. The RNase levels in human organs and body fluids were measured by enzyme immunoassay. In milk, semen and saliva, only RNase UL-like enzyme was found. Both RNase Us- and RNase UL-hke enzymes were found in kidney, stomach, and pancreas and the RNase Us/RNase UL ratios were 0.49, 1.35, and 0.34, respectively. In lung, liver, spleen, and leukocytes, most of the RNase activity was accounte