Add to ORKGA simple, general method for purifying restriction endo-nucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently link-ed to agarose. Most of the sixteen enzymes tested could be pur-ified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography
Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific si...
Although there are a number of protocols and commercial products available for isolating DNA fragmen...
Restriction endonuclease EcoRII (EcoRII) is a homodimeric DNA-binding protein. It belongs to the typ...
An abbreviated procedure has been developed for the purification of restriction endonucleases. This ...
A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, D...
The discovery of specific restriction e donucleases (Smith and Wilcox 1970) made possible the isolat...
the residual material in an appropriate buffer for subsequent studies. RNA will be isolated along wi...
The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucl...
A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is ...
The object of investigation: the microorganisms, ferments, nucleic acids. The purpose of the work: t...
Advanced Biotechnological research is largely depended on the genome analysis and recombinant DNA t...
An affinity chromatography method, using double stranded DNA, is described for the isolation of a de...
The ability of 223 Type II restriction endonucle-ases to hydrolyze RNA–DNA heteroduplex oligo-nucleo...
Digestion of DNA with restriction endonucleases is the first step in many gene manipulation projects...
A rapid and efficient method to purify λgt11 DNA is described. This technique involves precipitation...
Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific si...
Although there are a number of protocols and commercial products available for isolating DNA fragmen...
Restriction endonuclease EcoRII (EcoRII) is a homodimeric DNA-binding protein. It belongs to the typ...
An abbreviated procedure has been developed for the purification of restriction endonucleases. This ...
A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, D...
The discovery of specific restriction e donucleases (Smith and Wilcox 1970) made possible the isolat...
the residual material in an appropriate buffer for subsequent studies. RNA will be isolated along wi...
The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucl...
A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is ...
The object of investigation: the microorganisms, ferments, nucleic acids. The purpose of the work: t...
Advanced Biotechnological research is largely depended on the genome analysis and recombinant DNA t...
An affinity chromatography method, using double stranded DNA, is described for the isolation of a de...
The ability of 223 Type II restriction endonucle-ases to hydrolyze RNA–DNA heteroduplex oligo-nucleo...
Digestion of DNA with restriction endonucleases is the first step in many gene manipulation projects...
A rapid and efficient method to purify λgt11 DNA is described. This technique involves precipitation...
Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific si...
Although there are a number of protocols and commercial products available for isolating DNA fragmen...
Restriction endonuclease EcoRII (EcoRII) is a homodimeric DNA-binding protein. It belongs to the typ...