To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI Gm6ATC motif common in the genomic DNA of many bacteria including c-proteobacteria. Using synthetic genome mixtures, we demonstrate 80 % recovery of Escherichia coli genomic DNA even when only femtogram quantities are spiked into 10 mg of human DNA background. Binding is very specific with less than 0.5 % of human DNA bound. Next Generation Sequencing of input and enriched synthetic mixtures results in over 100-fold enrichment of target genomes relati...
Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible...
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized mode...
Identification of bacterial DNA consists from several steps: cell lysis, isolation and purification ...
<div><p>To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction e...
DNA samples derived from vertebrate skin, bodily cavities and body fluids contain both host and micr...
<div><p>DNA samples derived from vertebrate skin, bodily cavities and body fluids contain both host ...
Maps of genomic or cloned DNA are frequently constructed by analyzing the cleavage patterns produced...
Analysis of recently sequenced microbial genomes has revealed many DNA sequences that code for previ...
Recent advances in microbiome sequencing have rendered new insights into the role of the microbiome ...
ABSTRACT In sequenced genomes of prokaryotes, anomalous DNA (aDNA) can be recognized, among others, ...
Fundamental to many bacterial genome mapping strategies currently under development is the need to c...
Summary: Metagenomic sequencing is a promising approach for identifying and characterizing organisms...
Restriction digests were performed by incubating genomic DNA in the presence of the restriction endo...
Current large scale genome mapping methodology suffers from a lack of tools for generating specific ...
Mrr superfamily of homologous genes in microbial genomes restricts modified DNA in vivo. However, th...
Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible...
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized mode...
Identification of bacterial DNA consists from several steps: cell lysis, isolation and purification ...
<div><p>To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction e...
DNA samples derived from vertebrate skin, bodily cavities and body fluids contain both host and micr...
<div><p>DNA samples derived from vertebrate skin, bodily cavities and body fluids contain both host ...
Maps of genomic or cloned DNA are frequently constructed by analyzing the cleavage patterns produced...
Analysis of recently sequenced microbial genomes has revealed many DNA sequences that code for previ...
Recent advances in microbiome sequencing have rendered new insights into the role of the microbiome ...
ABSTRACT In sequenced genomes of prokaryotes, anomalous DNA (aDNA) can be recognized, among others, ...
Fundamental to many bacterial genome mapping strategies currently under development is the need to c...
Summary: Metagenomic sequencing is a promising approach for identifying and characterizing organisms...
Restriction digests were performed by incubating genomic DNA in the presence of the restriction endo...
Current large scale genome mapping methodology suffers from a lack of tools for generating specific ...
Mrr superfamily of homologous genes in microbial genomes restricts modified DNA in vivo. However, th...
Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible...
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized mode...
Identification of bacterial DNA consists from several steps: cell lysis, isolation and purification ...