Detection P53, Rb1 and H-ras Loss of Heterozygosity LOH in Patients with Urinary Bladder carcinoma

In this study 22 samples of urine sediments were obtained from patients with Urinary Bladder Carcinoma, DNA base technique and LOH detection was investigated in three genes P53, Rb1 and H-ras. The result are shown that LOH was present in most cases and for all genes where LOH in P53 represents (31.8%) and for Rb1 (45.4%) and for H-ras gene (27.2%).In some cases, more than one gene locus mutations were found. Bladder cancer is the second most common malignancy affecting the urinary system. Approximately 90 % of urothelial tumours are urothelial cancers (UCs). The remaining tumours are squamous cell cancers or adenocarcinomas. Small-cell carcinomas account for less than 1%. The spectrum of bladder cancer includes non–muscle-invasive, muscle-invasive, and metastatic disease, each with its own specific typical behaviour