Ellagic Acid in Root Bark of Oroxylum indicum by RP- HPLC with UV Detection

Oroxylum indicum (Syonakh), belonging to the family Bignoniaceae, has been used for the present study. Preliminary phytochemical screening revealed the presence of phytocostituents like flavonoids, alkaloids, tannins and anthraquinone in the root bark of Oroxylum indicum. The preliminary screening using TLC technique reflected the presence of four phytoconstituents such as baicalein, chrysin, biochanin-A and ellagic acid in both petroleum ether and hydrolysed n-butanol fractions. Modern phytochemical analysis in terms of quantification of different bioactive phytoconstituents in the root bark of Oroxylum indicum was performed using reverse phase high performance liquid chromatography (RP-HPLC) fingerprint. To evaluate the quality of root bark of Oroxylum indicum, a simple rapid and accurate RP-HPLC method was developed for the assessment of four bioactive phytoconstituents viz. chrysin, baicalein, biochanin-A and ellagic acid. The components were quantified in different extracts viz. alcohol, petroleum ether, ethyl acetate and n-buatnol successively. Quantification of phytoconstituents was done by using RP-HPLC in petroleum ether and hydrolysed n-butanol fraction. Standard baicalein, chrysin biochanin-A and ellagic acid (Sigma-Aldrich) wer