International Journal of Bioassays (IJB) 144 CHARACTERIZATION OF XYLANOLYTIC PAENIBACILLUS STRAINS ISOLATED IN THAILAND

Abstract: Seven xylanolytic bacteria were isolated from soils and muddy shore sediments collected in Thailand. All isolates were Gram-positive, spore-forming, rod-shaped bacteria. They were belonged to genus Paenibacillus and were divided into 5 groups based on their phenotypic and chemotaxonomic characteristics including 16S rRNA gene sequence analyses. They contained meso-diaminopimelic in cell wall peptidoglycan and menaquinone with seven isoprene units. An isolate (Group 1) was identified as P. macerans, 3 isolates (Group 2) were P. barengoltzii, each isolate in Group 3, 4 and 5, was P. timonensis, P. montaniterrae and P. dendritiformis, respectively. The xylanase producing isolate in Group 1 showed clear zone with 6.5 mm in diameter and produced xylanase activity with 3.20±0.7 unit/ml while Group 2 to Group 5 isolates showed clear zone with 1.5-5.0 mm in diameter and produced xylanase activity ranged from 1.07±0.15 to 1.86±0.20 unit/ml