DEVELOPMENT OF MIBI KIT FOR HEART IMAGING XA9847992

99mTc-isonitriles have been shown to be a very promising substitute for Thallium-201 (201TI) for myocardial perfusion imaging. In this study, the lyophillized kit of Methoxyisobutylisonitrile (MIBI) was prepared and labeled with 99mTc. Several factors affecting the labeling yield such as the kit's stannous content, boiling time during labeling, and the volume of 99mTc used during reconstitution were also investigated. The radiochemical purity (RCP) determination of the labeled product was analyzed by HPLC, solvent-extraction, TLC, and ITLC-SG chromatographic methods in various systems. Animal biodistribution study performed in rats indicated the "mTc-MIBI accumulation in the myocardial is up to 3 hours with little or no redistribution. Toxicity studies performed indicate no clinical signs of abnormality in mice at injected dose equivalent in amount of 100 times the human dose in proportion to body weight. Stability studies of the labeled complex performed at room temperature showed no change in radiochemical purity (> 95%) 6 hours post-preparation. Compatibility and comparative studies were done using both MINT and commercially available MIBI kits and 99mTc generator eluates. From the results obtained the MINT produced MIBI kits were found to be comparable in quality to that of commercials