A STEDmicroscope designed for routine biomedical applications. Prog Electromagn Res. 2014; 147:57–68. doi: 10.2528/PIER14042708

Abstract—We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales. 1