Antiviral Properties of Polyinosinic Acids containing Thio and Methyl Substitutions

Polyinosinic acids containing methyl and sulphur substitutions are potent inhibitors of reverse transcriptase. Substitution of sulphur for oxygen at the 6 position produces significant effects on the properties of polyinosinic acid: the kinetics of inhibition change from competitive to mixed-type and the inhibition constant falls by three orders of magnitude. In contrast, 1-methyl substitution produces no such effects. Poly(1-methyl-6-thioinosinic acid) or poly(mls6I) inhibits irreversibly, inhibiting all ten reverse transcriptases tested under a variety of assay conditions. In cell culture test systems, poly(mls6I) is capable of blocking both infection by non-transforming viruses and transformation by a sarcoma virus. The presence of poly(mls6I) in a preinfected culture results in the production of non-infectious virus particles lacking reverse t anscriptase activity