Associations of the porcine immune response and genetic polymorphisms with the shedding of Salmonella enterica serovar

A major focus of our collaborattve research 1s to 1nvest1gate the porctne response to Infection w1th Salmonella to 1) identify porctne genes differentially regulated during Infection and 2) Identify and associate genetic polymorphisms within these genes with infect1on status across swine populat1ons In the current study, 40 crossbred p1gs were intranasally Inoculated with Salmonella enterica serovar Typh1murium and mon1tored for Salmonella fecal shedding and blood 1mmune parameters at 2, 7, 14 and 20 days post-inoculation (dpi). Using a multivariate permutation test, a posit1ve correlation was observed between Salmonella shedding and Interferon-gamma (IFNG) levels at 2 and 7 dpi (p<0.05), w1th a greater number of Salmonella shedding 1n the an1mals w1th higher IFNG levels In addit1on, a positive correlation was observed between IFNG levels and the number of c1rculat1ng neutroph1ls at 7 and 14 dpi, mature banded neutroph1ls at 2 dp1, monocytes at 7 dp1 and wh1te blood cells (WBCs) at 7, 14 and 20 dp1. We have further performed assoc1atton studies of immune response parameters or shedding status of the Salmonella-Infected p1gs with single nucleotide polymorphisms (SNPs) in 9 genes · VCP, CCT7, LCP1, C047, SCARB2, CD163, CCR1, TLR4 and TYROBP Express1on of these genes was 1dent1fied by our group a