Cyclic adenosine 3',5'-monophosphateinduced ovulation in the perfused rat ovary and its mediation by prostaglandins

The role and mechanism of action of cyclic adenosine 3’,5’-monopbosphate (cAMP) in the ovulatory process was investigated by using the in vitro-perfused rat ovary model. Ovaries of pregnant mare’s serum gonad otropin (PMSG, 20 IU)-primed rats were perfused for 21 h beginning in the morning of induced proestrus. In vitro stimulation with luteinizing hormone (LH; 0.1 jig/mI) resulted in 2.4 ± 0.7 ovulations per treated ovary. Ovula-tions could also be induced by the addition of forskolin (30 pM) or dibutytyl cAMP (dbcAMP, 1 mM) with isobutylmethylxanthine (IBMX, 0.2 mM), with 11.8 ± 1.9 and 18.6 ± 4.4 ovulations per treated ovary, respec-tively. Indomethacin (5 pg/ml) significantly decreased the number of ovulations in the forskolin and dbcAMP # IBMX groups. The addition ofprostaglandin E2 (PGE2; 1 pg/mi three times during the perfusion) to the forskolin + indomethacin group reversed the inhibition of ovulation (21.6 ± 5.4 ovulations per treated ovary). Ovarian PGE tissue levels were significantly higher 10 b after stimulation with either LH, forskoiin, or dbcAMP + IBMX compared to the unstimulated control group. Ovulated oocytes in the LHandforskolin groups resumed meiosis but oocytes in the dbcAMP + IBMX groups remained immature. This study shows that an increase in ovarian cAMP, even if not induced by LH, is sufficient to cause ovulation of preovulatory rat follicles, supporting the involvement of cAMP in the normal ovulatory process of the PMSG-treated rat. Furthermore, prostaglandin involvement in cAMP-induced ovulations is demonstrated