New Bioactive Metabolites Produced by Phomopsis cassiae, an Endophytic Fungus in Cassia spectabilis

Dois novos metabólitos, 2,4-diidroxi-5,6-dimetil benzoato de etila (1) e phomopsilactona (2) foram isolados de Phomopsis cassiae, um fungo endofítico de Cassia spectabilis. As estruturas destes compostos foram elucidadas por dados espectrométricos de 1D e 2D RMN, EM e IV. As substâncias 1 e 2 exibiram forte atividade antifúngica contra os fungos fitopatogênicos Cladosporium cladosporioides e C. sphaerospermum, bem como citotoxidade contra a linhagem celular de tumor cervical humano (HeLa), em experimentos in vitro. Two new metabolites, ethyl 2,4-dihydroxy-5,6-dimethylbenzoate (1) and phomopsilactone (2) were isolated from Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. Their structures were elucidated by 1D and 2D NMR, MS and IR spectral data. Compounds 1 and 2 displayed strong antifungal activity against the phytopatogenic fungi Cladosporium cladosporioides and C. sphaerospermum, as well as cytotoxicity against human cervical tumor cell line (HeLa), in in vitro assays