DEVELOPMENT OF A REVERSED PHASE- HPLC METHOD FOR DETERMINATION OF MELOXICAM IN TABLET FORMULATION AND HUMAN SERUM

Developing a simple, economic, sensitive and rapid isocratic RP-HPLC method for determination of meloxicam (MX) in bulk drug, tablet formulation and human serum. The retention time observed for meloxicam was just 2.35 minutes using C18 column (150 x 3.0 mm, 5 μm) with a mobile phase consisting of acetonitrile: 0.2 % formic acid (70:30 v/v%) at a flow rate of 1 ml/min with UV detector set at 355 nm. Linearity in concentration range of 0.05 – 50 µg/ml, with coefficient of determination, R = 0.9956; slope= 25464 and intercept =-8872. The limit of detection and the limit of quantification were found to be 0.011 and 0.173 μg/ml, respectively. The precision and accuracy of method were checked by calculating RSD % and relative error E%, which were found to be reasonable. A RSD % (0.06 and 0.021%) for marketed brand and human serum, respectively and E % (0.64 and-0.47 %) for marketed brand and human serum, respectively. The method was found to be applicable for the analysis of MX in bulk, tablet formulation and human serum