Plasma Clearance and Oxidation of Dodecanedioic Acid in Humans

ABSTRACT. Background: Dicarboxylic acids are water-soluble, contrary to monocarboxylic acids, and have a metabolic path-way intermediate between those of lipids and carbohydrates. Our goal was to investigate the plasma turnover and oxidation rate of dodecanedioic acid (C12) in eight healthy male volunteers. Methods: A simultaneous infusion of both cold (0.24 mmol/ min corresponding to 0.396 kcal/min) and radiolabeled (1.62 μCi/min) C12 free acid was performed. Blood specimens were sampled over a period of 360 minutes, and 24-hour urine samples were collected to measure the levels of C12 by high-performance liquid chromatography and liquid scintillation. Indirect calorimetry was continuously performed, and expired 14CO2 was collected. Binding of C12 in human plasma was deter-mined in separate experiments using equilibrium dialysis. Results:A linear one-compartment model was used to describ