Persistence of human immunodeficiency virus (HIV) type 1 DNA in peripheral blood despite prolonged suppression of plasma HIV-1 RNA in children

Human immunodeficiency virus (HIV) type 1 DNA in peripheral blood mononuclear cells (PBMC) was quantified in 31 children who received efavirenz, nelfinavir, and 1 or 2 nucleoside reverse-transcriptase inhibitors for>2 years and in whom undetectable plasma HIV-1 RNA levels (,50 copies/mL) were sustained, to determine the usefulness of HIV-1 DNA as a marker of virus suppression. The median baseline HIV-1 DNA level was 750 copies/106 PBMC. After initiation of highly active antiretroviral therapy (HAART), HIV-1 DNA levels decreased gradu-ally, reaching a plateau from week 80 through week 104 (median HIV-1 DNA level, 263 copies/ 106 PBMC). Children who had plasma HIV-1 RNA levels,50 copies/mL after receiving HAART for 8 weeks (n 16) had persistently lower quantities of intracellular HIV-1 DNA than children whose HIV-1 RNA levels reached,50 copies/mL after 8 weeks of HAART (n 15). The median half-life for intracellular HIV-1 DNA was 60 weeks. Thus, despite prolonged maintenance of undetectable levels of plasma HIV-1 RNA, HIV-1 DNA remains detectable in PBMC of children and may be a useful marker of further virus suppression. The increased availability of antiretroviral agents that are active against human immunodeficiency virus (HIV) type 1 has made i