volume 10 Number 10 1982 Nucleic Acids Research Nucleotide sequence definition of a major human repeated DNA, the Hind HI 1.9 kb family

A human Hind III 1.9 kb repeated DNA fragment was isolated and cloned in pBR322. A cloned member that hybridized predominantly to the 1.9 kb Hind III band in a digest of whole human DNA was chosen for sequencing. It is an 1894 bp fragment that shows no significant internal repeats. Few pCG resi-dues are observed in the sequence and there are numerous stop codons. De-tailed sequence comparisons confirm this is a novel class of repeats that is not related to previously characterized human satellite DNAs or Alu se-quences. At least a portion of the sequence described is conserved in evolution