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Complications resulting from vascular disease such as atherosclerosis are a major health concern in the United States. Treatments to remove atherosclerotic plaques from occluded blood vessels often result in injury causing aberrant vascular smooth muscle cell (VSMC) proliferation leading to restenosis and vessel occlusion, rendering the procedure ineffective. Methods to prevent restenosis are limited due to the lack of understanding of the signaling pathways involved. The Notch signaling pathway is known to be important in VSMC development and likely contributes to vascular diseases, such as atherosclerosis and arteriosclerosis, and may be involved in the restenosis process. This study will define the actions of Notch signaling, and its interaction with two prominent signaling pathways, Platelet Derived Growth Factor (PDGF), which promotes proliferation and dedifferentiation and Transforming Growth Factor Beta (TGFß), which induces differentiation of VSMC. To investigate Notch signaling in vascular cells, human aortic smooth muscle cells were cultured and treated with pathway activators and inhibitors. RNA and protein was isolated from cultured cells and Western blots and quantitative polymerase chain reactions were used to measure the effects on smooth muscle differentiatio