Use of [3-3H]glucose and [6-14C]glucose to measure glucose turnover and glucose metabolism in humans

[ 3-3H] glucose and [ 6J4C]glucose to measure glucose turnover and glucose metabolism in humans. Am. J. Physiol. 263 (Endocrinol. Metab. 26): E17-E22, 1992.-[3-3H]glucose is fre-quently used to measure glucose turnover in humans. If fructose 6-phosphate-fructose 1,6-diphosphate cycling (Fpc) is negligible in both liver and muscle, then [3-3H]- and [6-14C]glucose (cor-rected for Cori cycle activity) should provide equivalent mea-sures of glucose turnover. In addition, if glycogenolysis is fully suppressed, then [ 14C]lactate specific activity should equal that of [6J4C]glucose from which it was derived, and oxidation of [6J4C]glucose, as measured by rate of generation of 14C02, should equal total glucose oxidation (i.e., that derived from in-tra- and extracellular pools) as measured by indirect calorimetry. To address these questions, glucose turnover was measured simultaneously with [3-3H]- and [6J4C]glucose in th