Bulletin USAMV-CN, 63/2007 DETERMINATION OF SULFONAMIDES IN CHICKEN MEAT BY SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract. This paper describes a method for the simultaneous detection and quantification of six sul-fonamides in chicken meat using normal phase cartridge clean-up and HPLC with UV detection. A liquid – liquid extraction and Sep- Pak silica clean-up procedure which minimizes the presence of halogenated solvents was used for sample preparation. The HPLC determination was performed using a RP C 18 column and sulfonamides were detected at 266 nm. Mobile phase was 0.01 M ammonium acetate pH 4.6 ( A) and methanol ( B). Chromatographic separation was obtained by gradient elution ( 22 % B to 50 % within 17 min, back to 22 % in 2 min, equilibration for 5 min).Average recoveries of analytes from spiked meat were higher than 74 %