Pr inted in Great Britain Characterization of Murine Sarcoma Virus (KmSTEN) Transformation of Mouse and Human Cells

rClRSTEN sarcoma virus induces foci of morphologically altered ceils in rat, mouse, and human cells in tissue culture. The KmSTEN sarcoma virus stock contains two viruses; one forming foci and the other plaques on XC cells. The sarcoma virus particle transforms cells in the absence of murine Ieukaemia virus but requires murine leukaemia virus for its replication. The high susceptibility of human cells to transformation by KmSTEN sarcoma virus is related to a function provided to the sarcoma virus by murine leukaemia virus. A large number of KIRSTEN sarcoma virus-transformed mouse and rat cell loci has been isolated, and from these many non-producer clonal lines have been derived and characterized