Interaction of 4-Nitroquinoline 1-Oxide with Deoxyribonucleic Acid and Synthetic Polydeoxyribonucleotides

Native calf thymus DNA and, to a lesser extent, poly dG: dC produced marked changes in the absorption spectrum of 4-nitroquinohine 1-oxide (4-NQO) as determined by difference spectrum measurements. Poly d(A-T) or denatured calf thymus DNA caused minor chuanges in the absorption spectrum of 4-NQO, using difference spectrum methods. The addition of sodium chloride from 0.5 mi to 1.0 M decreased the effect of native DNA on tlue difference spectrum of 4-NQO by approximately 4-fold. The interaction was par-tially dependent upon ionic parameters, which suggested the importaiuce of charged sites in the interaction of 4-NQO with DNA. Urea (6 i) abolished the effect of DNA on the difference spectrum of 4-NQO. The effect of urea may indicate some role of hydrogen bonding in the formation of the DNA-4-NQO complex or in the alteration of hydrophobic interactions resulting in disruption of the DNA-4-NQO complex. Strand separation (Tm) of native DNA i, significantly stabilized by the addition of 4-NQO. Studies on thue binding of native DNA with 4-NQO resulted in a nonlinear curve, consistent with the involvement of more than one site of interaction in the formation of the DNA-4-NQO complex. The integrity of the double-helical cotuformation of DNA and the presence of G-C urs appeared to be essential for maximal complex formation. The binding of 4-NQO to 1)XA evidently is complex and cannot be described by a single model of complex formation