Complete Genome Sequence of Clostridium sp. Strain BNL1100, a Cellulolytic Mesophile Isolated from

We present the full genome sequence of Clostridium sp. strain BNL1100, a Gram-positive, endospore-forming, lignocellu-lolytic bacterium isolated from a corn stover enrichment culture. The 4,613,747-bp genome of strain BNL1100 contains 4,025 putative protein-coding genes, of which 103 are glycoside hydrolases, the highest detected number in cluster III clostridia. The discovery and exploration of novel cellulolytic clostridia areessential for the development of technologies for the direct conversion of lignocellulosic biomass to fuels (6, 7). Here we report whole-genome sequencing of Clostridium sp. strain BNL1100, a novel Gram-positive, endospore-forming, lignocellu-losic bacterium most closely related to Clostridium cellulolyticum and Clostridium papyrosolvens. The genome information will be useful for exploring Clostridium sp. BNL1100 as a biomass-de-composing microorganism for biofuel production. Clostridium sp. BNL1100 was isolated from a corn stover enrichment culture. Genome sequencing was performed using a combination of Illumina (1) and 454 technologies (8) by th