Original Article Increased Dietary Substrate Delivery Alters Hepatic Fatty Acid Recycling in Healthy Men

Sources of fatty acids flowing to the liver may be used for triacylglycerol (TAG) synthesis. Our objective was to quantify contributions of nonesterified fatty acids (NEFAs), de novo lipogenesis, and dietary fatty acids to VLDL-TAG in the fed state after meal feeding in healthy subjects (n 6). The effect of substrate delivery rate was also determined by comparison with data obtained under a continuous-feeding regimen. A liquid diet was administered by mouth or via feeding tube. Contribu-tions of NEFAs, de novo lipogenesis, and dietary fatty acids to VLDL-TAG were quantified using stable iso-topes and gas chromatography–mass spectrometry. Con-tribution of NEFAs to VLDL-TAG was similar under meal feeding and continuous feeding, although insulin area under the curve (AUC) was greater under mea