Bulletin USAMV-CN, 63/2007 ANALYSIS OF GENETIC VARIABILITY AT MITOCHONDRIAL DNA LEVEL IN SOME ROUMANIAN PHYTOPHTHORA INFESTANS ACCESSIONS

haplotypes. Abstract: Analysis of secondary molecular polymorphism at the level of amplified mitochondrial DNA, digested by restriction enzymes (CAPS markers), revealed genetic differentiation among ten Phytophthora infestans accessions. Mitochondrial DNA was amplified with four pairs of primers (H1,H2,H3 and H4) and after that the products of amplification were digested with Hha I (for H1 products of amplification), Msp I (for H2 products of amplification) and Eco R I (for H3 and H4 products of amplification) restriction enzymes. On this basis we have established that the ten Phytophthora infestans accessions belongs to two haplotypes, one accession belongs to Ia haplotyp and the others belong to IIa haplotype