A SEMI-QUANTITATIVE METHOD FOR DETERMINING THE LEVELS OF CARBONIC ANHYDRASE ISOZYMES

A procedure is presented which can be used to analyse the isozymes of carbonic anhydrase (CA I and CA II) as well as other specific soluble proteins of individual erythrocytes. The concentration of red cell CA I in the pig-tailed macaque (Macaca nemestrina) was estimated by the technique employed, as well as the relative concentration in individual cells. In addition, the basic technique was modified to include electrophoresis of the carbonic anhydrases of the individual cells. Recently Gitlin et al. [l] and Daufi & Ron- the method of Gitlin et al. [l] has been shown dell [2] independently reported immuno- to be useful for quantitation, it does not chemical methods for the analysis of carbonic allow direct visualization of both the cell anhydrase and other specific soluble proteins and its contents at the same time. The method in individual human red blood cells. Although of Daufi & Rondell [2] has not been shown-_ _ to be quantitative; however, it does permit 1 Present address: Fact&ad de Medicina, Hospital de San Pablo, Universidad Autonoma de Barcelona, direct visualization and we feel this attribut