iV-Pepstatinyl-iV'-dansyldiaminopropane (dansyl-pepstatin) was prepared by the cou-pling of pepstatin A and iV-dansyl-diaminopropane. The dansyl-pepstatin obtained strongly inhibited pepsin activity by forming a 1:1 complex. The fluorescence of the dansyl group (excitation at 320 nm, and emission near 520 nm) increased with the formation of the complex. The increase in fluorescence of dansyl-pepstatin solution was proportional to the amount of added pepsin, chymosin and cathepsin D until dansyl-pepstatin was saturated by these enzymes and at higher protease concentrations the fluorescence did not increase further. Therefore, the net amounts of active pepstatin-sensitive carboxyl proteases could be determined by detecting the inflection...
The ubiquitin-proteasome pathway is a major route of degradation of cell proteins. It also plays an ...
Potato chymotrypsin inhibitor-1 (pCTI-1) was biotinylated by reaction with sulfosuccinimidyl-6-(biot...
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are pro...
The fluorescence of pepsin-bound 2-p-toluidinylnaphthalene-6-sulfonate (TNS) decreases upon the bind...
ABSTRACT: Thioamide quenchers can be paired with compact fluorophores to design “turn-on ” fluoresce...
An intramolecularly quenched fluorogenic peptide containing o-aminobenzoyl (Abz) and ethylenediamine...
SummaryThe determination of enzyme activity or inhibition in intact living cells is a problem in the...
The determination of enzyme activity or inhibition in intact living cells is a problem in the develo...
Thioamide quenchers can be paired with compact fluorophores to design “turn-on” fluorescent protease...
Analogues of the acid protease inhibitor, pepstatin (9), have been synthesised and tested for inhibi...
International audienceThe light chain of tetanus toxin (TeNT-L chain), endowed with a zinc metalloen...
Anomalous protease activities are associated with many diseases. Great efforts are paid for selectin...
The activity concentration of pepsin may be quantified by using azocoll as a chromogenic substrate.T...
228 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.In order to study the role of...
We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the de...
The ubiquitin-proteasome pathway is a major route of degradation of cell proteins. It also plays an ...
Potato chymotrypsin inhibitor-1 (pCTI-1) was biotinylated by reaction with sulfosuccinimidyl-6-(biot...
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are pro...
The fluorescence of pepsin-bound 2-p-toluidinylnaphthalene-6-sulfonate (TNS) decreases upon the bind...
ABSTRACT: Thioamide quenchers can be paired with compact fluorophores to design “turn-on ” fluoresce...
An intramolecularly quenched fluorogenic peptide containing o-aminobenzoyl (Abz) and ethylenediamine...
SummaryThe determination of enzyme activity or inhibition in intact living cells is a problem in the...
The determination of enzyme activity or inhibition in intact living cells is a problem in the develo...
Thioamide quenchers can be paired with compact fluorophores to design “turn-on” fluorescent protease...
Analogues of the acid protease inhibitor, pepstatin (9), have been synthesised and tested for inhibi...
International audienceThe light chain of tetanus toxin (TeNT-L chain), endowed with a zinc metalloen...
Anomalous protease activities are associated with many diseases. Great efforts are paid for selectin...
The activity concentration of pepsin may be quantified by using azocoll as a chromogenic substrate.T...
228 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.In order to study the role of...
We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the de...
The ubiquitin-proteasome pathway is a major route of degradation of cell proteins. It also plays an ...
Potato chymotrypsin inhibitor-1 (pCTI-1) was biotinylated by reaction with sulfosuccinimidyl-6-(biot...
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are pro...